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triton x 100 thermo fisher scientific cat  (Thermo Fisher)


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    Thermo Fisher triton x 100 thermo fisher scientific cat
    Triton X 100 Thermo Fisher Scientific Cat, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermo+fisher+scientific+cat/Triton+X-100/pm42323894-22-135-137
    Average 99 stars, based on 1 article reviews
    triton x 100 thermo fisher scientific cat - by Bioz Stars, 2026-09
    99/100 stars

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    Transformation Assay:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Cloning:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Plasmid Preparation:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Mutagenesis:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Protein Purification:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Biomarker Discovery:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Purification:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Staining:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Article Title: TGM1/3-mediated transamidation of Exo70 promotes tumor metastasis upon LKB1 inactivation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-FLAG antibody Sigma-Aldrich Cat. #F1804; RRID:AB_262044 anti-Tubulin antibody Sigma-Aldrich Cat. #T8328; RRID:AB_1844090 anti-b-actin antibody Sigma-Aldrich Cat. #A3854; RRID:AB_262011 anti-Exo70 antibody Abcam Cat. #ab118792; RRID:AB_10901372 anti-Myc antibody Roche Cat. #11667149001; RRID:AB_390912 anti-LKB1 antibody Santa Cruz Biotechnology Cat. #sc-32245; RRID:AB_627890 anti-Exo70 antibody Santa Cruz Biotechnology Cat. #sc-365825; RRID:AB_10843358 anti-HA antibody Proteintech Cat. #51064-2-AP; RRID:AB_11042321 anti-TGM1 antibody Proteintech Cat. #12912-3-AP; RRID:AB_2271748 anti-Cystatin A antibody Proteintech Cat. # 15962-1-AP; RRID:AB_10641043 anti-TGM3 antibody Sangon Biotech. .. Cat. #D221330; RRID:AB_3224413 anti-Integrin b1 antibody Cell Signaling Technology Cat. #34971; RRID:AB_2799067 Normal Rabbit IgG antibody Cell Signaling Technology Cat. #2729; RRID:AB_1031062 goat anti-mouse IgG secondary antibody (Alexa Fluor 488 conjugate) Thermo Fisher Scientific Cat. #A-11029; RRID:AB_2534088 goat anti-rabbit IgG secondary antibody (Alexa Fluor 594 conjugate) Thermo Fisher Scientific Cat. #A-11037; RRID:AB_2534095 HRP-conjugated Affinipure Goat AntiMouse IgG(H + L) Proteintech Cat. #SA00001-1; RRID:AB_2722565 HRP-conjugated Affinipure Goat AntiRabbit IgG(H + L) Proteintech Cat. # SA00001-2; RRID:AB_2722564 Biological samples Lung tissue samples The tissue bank of Zhongshan Hospital Affiliated to Xiamen University N/A Chemicals, peptides, and recombinant proteins Rhodamine Phalloidin Cytoskeleton Cat. #PHDR1 Fluoromount-G Southern Biotechnology Associates Cat. #0100-20 Phosbind Acrylamide APExBIO Technology Cat. #F4002 Cantharidin Targetmol Cat. #T2903 norcantharidin Targetmol Cat. #T2894 Okadaic acid potassium salt Beyotime Biotechnology Cat. #S1786 Matrigel Corning Cat. #354234 Beetle Luciferin Promega Cat. #E1605 Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat. #sc-2003 Prestained protein ladder Thermo Scientific Cat. #26617 Prestained DNA ladder Takara Cat. #3584A Critical commercial assays Pierce Glycoprotein Staining Kit Thermo Scientific Cat. #24562 Experimental models: Cell lines HEK293T Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMGNHu17 HeLa Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu187 HCT116 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu99 (Continued on next page) Cell Reports 43, 114604, August 27, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER SW620 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu101 RKO Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu116 NCI-1299 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMSCSP589 NCI-H460 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu205 A549 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMSCSP503 NCI-H1437 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMSCSP591 MCF-7 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMSCSP531 MDA-MB-231 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu227 HepG2 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu72 HS578T kindly provided by Professor Bo-an Li (Xiamen University, Xiamen, Fujian, China) N/A MGC-803 kindly provided by Professor Jianghua Yan (Xiamen University, Xiamen, Fujian, China) N/A A2058 kindly provided by Professor Jianghua Yan (Xiamen University, Xiamen, Fujian, China) N/A Experimental models: Organisms/strains nude mice (BALB/c) the SLAC Laboratory Animal Center, China N/A Oligonucleotides See Table S1 for the shRNA sequences for target genes N/A N/A See Table S2 for real-time PCR primers N/A N/A Recombinant DNA Exo70 (rattus) kept in our lab N/A Sec6 (human) kept in our lab N/A Sec15 (human) kept in our lab N/A Exo84 (human) kept in our lab N/A BRSK1 (human) https://doi.org/10.1038/nature09627 pDONR223-BRSK1, RRID:Addgene_23597 MARK2 (human) https://doi.org/10.1038/nature09627 pDONR223-MARK2, RRID:Addgene_23404 MARK3 (human) https://doi.org/10.1038/nature09627 pDONR223-MARK3, RRID:Addgene_23716 NUAK2 (human) https://doi.org/10.1038/nature09627 pDONR223-NUAK2, RRID:Addgene_23831 SIK1 (human) https://doi.org/10.1534/g3.118.200144 SIK1_pCSdest, RRID:Addgene_53936 LKB1, STRADa, MO25a, CaMKKb, AMPKa1, AMPKa2, AMPKb and AMPKg (all human) kindly provided by Professor Qiao Wu (Xiamen University, Xiamen, Fujian, China) N/A TGM1, TGM3, Ubiquitin and Cystatin A (all human) obtained by reverse transcription using RNA from HeLa cells N/A Software and algorithms Graphpad Prism Graphpad Software Inc., La Jolla, CA, USA https://www.graphpad.com Proteome Discoverer 2.5 software against Uniprot database Thermo Scientific, Waltham, MA, USA https://www.thermofisher.com 16 Cell Reports 43, 114604, August 27, 2024

    Activity Assay:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Recombinant:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Article Title: TGM1/3-mediated transamidation of Exo70 promotes tumor metastasis upon LKB1 inactivation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-FLAG antibody Sigma-Aldrich Cat. #F1804; RRID:AB_262044 anti-Tubulin antibody Sigma-Aldrich Cat. #T8328; RRID:AB_1844090 anti-b-actin antibody Sigma-Aldrich Cat. #A3854; RRID:AB_262011 anti-Exo70 antibody Abcam Cat. #ab118792; RRID:AB_10901372 anti-Myc antibody Roche Cat. #11667149001; RRID:AB_390912 anti-LKB1 antibody Santa Cruz Biotechnology Cat. #sc-32245; RRID:AB_627890 anti-Exo70 antibody Santa Cruz Biotechnology Cat. #sc-365825; RRID:AB_10843358 anti-HA antibody Proteintech Cat. #51064-2-AP; RRID:AB_11042321 anti-TGM1 antibody Proteintech Cat. #12912-3-AP; RRID:AB_2271748 anti-Cystatin A antibody Proteintech Cat. # 15962-1-AP; RRID:AB_10641043 anti-TGM3 antibody Sangon Biotech. .. Cat. #D221330; RRID:AB_3224413 anti-Integrin b1 antibody Cell Signaling Technology Cat. #34971; RRID:AB_2799067 Normal Rabbit IgG antibody Cell Signaling Technology Cat. #2729; RRID:AB_1031062 goat anti-mouse IgG secondary antibody (Alexa Fluor 488 conjugate) Thermo Fisher Scientific Cat. #A-11029; RRID:AB_2534088 goat anti-rabbit IgG secondary antibody (Alexa Fluor 594 conjugate) Thermo Fisher Scientific Cat. #A-11037; RRID:AB_2534095 HRP-conjugated Affinipure Goat AntiMouse IgG(H + L) Proteintech Cat. #SA00001-1; RRID:AB_2722565 HRP-conjugated Affinipure Goat AntiRabbit IgG(H + L) Proteintech Cat. # SA00001-2; RRID:AB_2722564 Biological samples Lung tissue samples The tissue bank of Zhongshan Hospital Affiliated to Xiamen University N/A Chemicals, peptides, and recombinant proteins Rhodamine Phalloidin Cytoskeleton Cat. #PHDR1 Fluoromount-G Southern Biotechnology Associates Cat. #0100-20 Phosbind Acrylamide APExBIO Technology Cat. #F4002 Cantharidin Targetmol Cat. #T2903 norcantharidin Targetmol Cat. #T2894 Okadaic acid potassium salt Beyotime Biotechnology Cat. #S1786 Matrigel Corning Cat. #354234 Beetle Luciferin Promega Cat. #E1605 Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat. #sc-2003 Prestained protein ladder Thermo Scientific Cat. #26617 Prestained DNA ladder Takara Cat. #3584A Critical commercial assays Pierce Glycoprotein Staining Kit Thermo Scientific Cat. #24562 Experimental models: Cell lines HEK293T Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMGNHu17 HeLa Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu187 HCT116 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu99 (Continued on next page) Cell Reports 43, 114604, August 27, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER SW620 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu101 RKO Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu116 NCI-1299 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMSCSP589 NCI-H460 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu205 A549 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMSCSP503 NCI-H1437 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMSCSP591 MCF-7 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMSCSP531 MDA-MB-231 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu227 HepG2 Cell Bank, Type Culture Collection, Chinese Academy of Sciences CSTR:19375.09.3101HUMTCHu72 HS578T kindly provided by Professor Bo-an Li (Xiamen University, Xiamen, Fujian, China) N/A MGC-803 kindly provided by Professor Jianghua Yan (Xiamen University, Xiamen, Fujian, China) N/A A2058 kindly provided by Professor Jianghua Yan (Xiamen University, Xiamen, Fujian, China) N/A Experimental models: Organisms/strains nude mice (BALB/c) the SLAC Laboratory Animal Center, China N/A Oligonucleotides See Table S1 for the shRNA sequences for target genes N/A N/A See Table S2 for real-time PCR primers N/A N/A Recombinant DNA Exo70 (rattus) kept in our lab N/A Sec6 (human) kept in our lab N/A Sec15 (human) kept in our lab N/A Exo84 (human) kept in our lab N/A BRSK1 (human) https://doi.org/10.1038/nature09627 pDONR223-BRSK1, RRID:Addgene_23597 MARK2 (human) https://doi.org/10.1038/nature09627 pDONR223-MARK2, RRID:Addgene_23404 MARK3 (human) https://doi.org/10.1038/nature09627 pDONR223-MARK3, RRID:Addgene_23716 NUAK2 (human) https://doi.org/10.1038/nature09627 pDONR223-NUAK2, RRID:Addgene_23831 SIK1 (human) https://doi.org/10.1534/g3.118.200144 SIK1_pCSdest, RRID:Addgene_53936 LKB1, STRADa, MO25a, CaMKKb, AMPKa1, AMPKa2, AMPKb and AMPKg (all human) kindly provided by Professor Qiao Wu (Xiamen University, Xiamen, Fujian, China) N/A TGM1, TGM3, Ubiquitin and Cystatin A (all human) obtained by reverse transcription using RNA from HeLa cells N/A Software and algorithms Graphpad Prism Graphpad Software Inc., La Jolla, CA, USA https://www.graphpad.com Proteome Discoverer 2.5 software against Uniprot database Thermo Scientific, Waltham, MA, USA https://www.thermofisher.com 16 Cell Reports 43, 114604, August 27, 2024

    Modification:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Article Title: An Orthotopic Vestibular Schwannoma Mouse Model to Study Tumor-Host Interactions and Mechanism of Sensorineural Hearing Loss.
    Article Snippet: .. At our institution, the cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; high glucose, with L-glutamine, sodium pyruvate, and phenol red; without HEPES) (Thermo Fisher Scientific, Cat. No. 11995073) supplemented with 10 % fetal bovine serum (FBS) (Thermo Fisher Scientific, Cat. No. 16000044), 1 % penicillin/streptomycin (Sigma-Aldrich, Cat. No. P4333) and 10 μM forskolin (Sigma-Aldrich, Cat. No. 344270) at 37◦C in a humidified atmosphere with 5 % CO2. .. Cell cultures were routinely tested for mycoplasma contamination (MycoAlert, Lonza Biosciences, Cat. No. LTO7).

    Expressing:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: Materials with vendor and catalog information Bacterial Strains and Competent Cells Source Identifier NEB 5-alpha Competent E. coli (High Efficiency) New England Biolabs Cat#C2987H BLR(DE3) Competent Cells Novagen Cat#69053 Mix & Go! .. E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific CatJ62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2. ..

    Saline:

    Article Title: Sialylated IgG induces the transcription factor REST in alveolar macrophages to protect against lung inflammation and severe influenza disease.
    Article Snippet: 155506; RRID: AB_2750234 BV421 anti-MerTK BioLegend cat. no. 151510; RRID: AB_2832533 APC/Fire-750 anti–major histocompatibility complex (MHC) II BioLegend cat. no. 107652; RRID: AB_2616728 APC anti-CD209b Invitrogen cat. no. 17-2093-82; RRID: AB_11151692 BUV395 anti-CD24 BD Horizon cat. no. 566221; RRID: AB_2632389 BUV805 anti-CD103 BD Horizon cat. no. 741948; RRID: AB_2871259 PE-Cy7 anti-CD32b BioLegend cat. no. 303213; RRID: AB_2616922 PEDazzle 594 anti-CD64 BioLegend cat. no. 305031; RRID: AB_2564186 BUV615 anti-CD16 BD Horizon cat. no. 751572; RRID: AB_2875567 Human anti-kappa Southern Biotech cat. no. 9230-01; RRID: AB_2796705 Human anti-lambda Southern Biotech cat. no. 9180-01; RRID: AB_2796674 HRP–conjugated anti-human IgG secondary antibody Southern Biotech cat. no. 2040-05: RRID: AB_2795644 PerCP-Cy5.5 anti-CD3 BioLegend cat. no. 100218; RRID: AB_1595492 PerCP-Cy5.5 anti-B220 BioLegend cat. no. 103236; RRID: AB_893354 APC-Fire750 anti-NK-1.1 BioLegend cat. no. 156516; RRID: AB_2892323 PE anti-CD11b BioLegend cat. no. 101207; RRID: AB_312790 APC anti-Ly6C BioLegend cat. no. 128016; RRID: AB_1732076 APC-Fire750 anti-Ly6C BioLegend cat. no. 128046; RRID: AB_2616730 BV785 anti-Ly6G BioLegend cat. no. 127645; RRID: AB_2566317 PE anti-myeloid-epithelial-reproductive tyrosine kinase (MerTK) BioLegend cat. no. 151506; RRID: AB_2617036 BV650 anti-human CD16 BioLegend cat. no. 302042; RRID: AB_2563801 APC/Fire 750 anti–I-A/I-E BioLegend cat. no. 107652; RRID: AB_2616728 BV785 anti–I-A/I-E BioLegend cat. no. 107645; RRID: AB_2565977 BV421 anti-CD16 BioLegend cat. no. 302038; RRID: AB_10898112 FITC anti-CD32a STEMCELL cat. no. 60012FI.1 APC anti-CD32b BioLegend cat. no. 398304; RRID: AB_2860997 PerCP-Cy5.5 anti-CD209 BioLegend cat. no. 330110; RRID: AB_1236468 rabbit anti-REST Invitrogen cat. no. MA5-44189; RRID: AB_2926319 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline Gibco cat. no. 14190-144 Non-fat Milk Lab Scientific bioKEMIX cat. no. M0841 SDS sample buffer Boster cat. no. AR1112 Single-cell lysis buffer Takara cat. no. 635013 RNase inhibitor Takara cat. no. 2313B Fixation Buffer BioLegend cat. no. 420801 DharmaFect3 Transfection Reagent Horizon Discovery cat. no. NC1650394 Non-essential amino acids Gibco cat. no. 11-140-050 Sodium pyruvate Gibco cat. no. 11-360-070 Macrophage-colony stimulating factor (M-CSF) Peprotech cat. no. 300-25 IL-4 Peprotech cat. no. 200-04 glycine Sigma-Aldrich cat. no. G8898 TritonX-100 Sigma-Aldrich cat. no. 93443 ProLong Gold Antifade Reagent Cell Signaling Technology cat. no. P36930 Normocin Invivogen cat. no. ant-nr-1 Blasticidin Invivogen cat. no. ant-bl-1 phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific cat. no. J63916.MCR lipopolysaccharide (LPS) eBioscience cat. no. 50-112-2025 QUANTI-Blue solution Invivogen cat. no. rep-qbs Hoechst 33342 Abcam Inc. cat. no. ab228551 Alexa Fluor 488 phalloidin Invitrogen cat. no. A12379 LIVE/DEAD Blue Fixable Dye Thermo Fisher Scientific cat. no. L34962 LIVE/DEAD Aqua Fixable Dye Thermo Fisher Scientific cat. no. L34957 Critical Commercial Assays Nano-Glo Luciferase Assay System Promega cat. no. N1120 Legendplex Bead array kits Biolegend cat. nos. .. 740390, 740451 RNeasy Micro Kit Qiagen cat. no. 74004 Gibson Assembly New England BioLabs cat. no. E2611L EasySep PE Positive Selection Kit II STEMCELL cat. no. 17684 SMART-Seq v4 Ultra Low Input RNA kit Takara cat. no. 634888 Nextera XT library preparation kit Illumina cat. no. FC-131-1096 Deposited Data Data from human PBMC of influenza patients from public datasets Liu et al.,55 Zhai et al.,56 Sweeney et al.,57 Wong et al.58–60 Ramilo et al.,61 Parnell et al.,62 Berdal et al.,63 Bermejo-Martin et al.,64 Parnell et al.,65 Zaas et al.,66 Dunning et al.,67 and Tang et al.68 GSE73072, GSE68310, GSE66099, GSE4607, GSE6269, GSE40012, GSE27131, GSE21802, GSE20346, GSE17156, GSE111368, GSE101702 Data for generating figures This paper Immport SDY2810 Experimental models: Cell lines MDCK cell BEI and Dr. Gene Tan N/A FreeStyle 293-F cells Thermo Fisher cat. no. R790-07 THP1-Blue NF-kB cells Invivogen cat. no. thp-nfkbv2 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e3

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Milk:

    Article Title: Sialylated IgG induces the transcription factor REST in alveolar macrophages to protect against lung inflammation and severe influenza disease.
    Article Snippet: 155506; RRID: AB_2750234 BV421 anti-MerTK BioLegend cat. no. 151510; RRID: AB_2832533 APC/Fire-750 anti–major histocompatibility complex (MHC) II BioLegend cat. no. 107652; RRID: AB_2616728 APC anti-CD209b Invitrogen cat. no. 17-2093-82; RRID: AB_11151692 BUV395 anti-CD24 BD Horizon cat. no. 566221; RRID: AB_2632389 BUV805 anti-CD103 BD Horizon cat. no. 741948; RRID: AB_2871259 PE-Cy7 anti-CD32b BioLegend cat. no. 303213; RRID: AB_2616922 PEDazzle 594 anti-CD64 BioLegend cat. no. 305031; RRID: AB_2564186 BUV615 anti-CD16 BD Horizon cat. no. 751572; RRID: AB_2875567 Human anti-kappa Southern Biotech cat. no. 9230-01; RRID: AB_2796705 Human anti-lambda Southern Biotech cat. no. 9180-01; RRID: AB_2796674 HRP–conjugated anti-human IgG secondary antibody Southern Biotech cat. no. 2040-05: RRID: AB_2795644 PerCP-Cy5.5 anti-CD3 BioLegend cat. no. 100218; RRID: AB_1595492 PerCP-Cy5.5 anti-B220 BioLegend cat. no. 103236; RRID: AB_893354 APC-Fire750 anti-NK-1.1 BioLegend cat. no. 156516; RRID: AB_2892323 PE anti-CD11b BioLegend cat. no. 101207; RRID: AB_312790 APC anti-Ly6C BioLegend cat. no. 128016; RRID: AB_1732076 APC-Fire750 anti-Ly6C BioLegend cat. no. 128046; RRID: AB_2616730 BV785 anti-Ly6G BioLegend cat. no. 127645; RRID: AB_2566317 PE anti-myeloid-epithelial-reproductive tyrosine kinase (MerTK) BioLegend cat. no. 151506; RRID: AB_2617036 BV650 anti-human CD16 BioLegend cat. no. 302042; RRID: AB_2563801 APC/Fire 750 anti–I-A/I-E BioLegend cat. no. 107652; RRID: AB_2616728 BV785 anti–I-A/I-E BioLegend cat. no. 107645; RRID: AB_2565977 BV421 anti-CD16 BioLegend cat. no. 302038; RRID: AB_10898112 FITC anti-CD32a STEMCELL cat. no. 60012FI.1 APC anti-CD32b BioLegend cat. no. 398304; RRID: AB_2860997 PerCP-Cy5.5 anti-CD209 BioLegend cat. no. 330110; RRID: AB_1236468 rabbit anti-REST Invitrogen cat. no. MA5-44189; RRID: AB_2926319 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline Gibco cat. no. 14190-144 Non-fat Milk Lab Scientific bioKEMIX cat. no. M0841 SDS sample buffer Boster cat. no. AR1112 Single-cell lysis buffer Takara cat. no. 635013 RNase inhibitor Takara cat. no. 2313B Fixation Buffer BioLegend cat. no. 420801 DharmaFect3 Transfection Reagent Horizon Discovery cat. no. NC1650394 Non-essential amino acids Gibco cat. no. 11-140-050 Sodium pyruvate Gibco cat. no. 11-360-070 Macrophage-colony stimulating factor (M-CSF) Peprotech cat. no. 300-25 IL-4 Peprotech cat. no. 200-04 glycine Sigma-Aldrich cat. no. G8898 TritonX-100 Sigma-Aldrich cat. no. 93443 ProLong Gold Antifade Reagent Cell Signaling Technology cat. no. P36930 Normocin Invivogen cat. no. ant-nr-1 Blasticidin Invivogen cat. no. ant-bl-1 phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific cat. no. J63916.MCR lipopolysaccharide (LPS) eBioscience cat. no. 50-112-2025 QUANTI-Blue solution Invivogen cat. no. rep-qbs Hoechst 33342 Abcam Inc. cat. no. ab228551 Alexa Fluor 488 phalloidin Invitrogen cat. no. A12379 LIVE/DEAD Blue Fixable Dye Thermo Fisher Scientific cat. no. L34962 LIVE/DEAD Aqua Fixable Dye Thermo Fisher Scientific cat. no. L34957 Critical Commercial Assays Nano-Glo Luciferase Assay System Promega cat. no. N1120 Legendplex Bead array kits Biolegend cat. nos. .. 740390, 740451 RNeasy Micro Kit Qiagen cat. no. 74004 Gibson Assembly New England BioLabs cat. no. E2611L EasySep PE Positive Selection Kit II STEMCELL cat. no. 17684 SMART-Seq v4 Ultra Low Input RNA kit Takara cat. no. 634888 Nextera XT library preparation kit Illumina cat. no. FC-131-1096 Deposited Data Data from human PBMC of influenza patients from public datasets Liu et al.,55 Zhai et al.,56 Sweeney et al.,57 Wong et al.58–60 Ramilo et al.,61 Parnell et al.,62 Berdal et al.,63 Bermejo-Martin et al.,64 Parnell et al.,65 Zaas et al.,66 Dunning et al.,67 and Tang et al.68 GSE73072, GSE68310, GSE66099, GSE4607, GSE6269, GSE40012, GSE27131, GSE21802, GSE20346, GSE17156, GSE111368, GSE101702 Data for generating figures This paper Immport SDY2810 Experimental models: Cell lines MDCK cell BEI and Dr. Gene Tan N/A FreeStyle 293-F cells Thermo Fisher cat. no. R790-07 THP1-Blue NF-kB cells Invivogen cat. no. thp-nfkbv2 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e3

    Single Cell:

    Article Title: Sialylated IgG induces the transcription factor REST in alveolar macrophages to protect against lung inflammation and severe influenza disease.
    Article Snippet: 155506; RRID: AB_2750234 BV421 anti-MerTK BioLegend cat. no. 151510; RRID: AB_2832533 APC/Fire-750 anti–major histocompatibility complex (MHC) II BioLegend cat. no. 107652; RRID: AB_2616728 APC anti-CD209b Invitrogen cat. no. 17-2093-82; RRID: AB_11151692 BUV395 anti-CD24 BD Horizon cat. no. 566221; RRID: AB_2632389 BUV805 anti-CD103 BD Horizon cat. no. 741948; RRID: AB_2871259 PE-Cy7 anti-CD32b BioLegend cat. no. 303213; RRID: AB_2616922 PEDazzle 594 anti-CD64 BioLegend cat. no. 305031; RRID: AB_2564186 BUV615 anti-CD16 BD Horizon cat. no. 751572; RRID: AB_2875567 Human anti-kappa Southern Biotech cat. no. 9230-01; RRID: AB_2796705 Human anti-lambda Southern Biotech cat. no. 9180-01; RRID: AB_2796674 HRP–conjugated anti-human IgG secondary antibody Southern Biotech cat. no. 2040-05: RRID: AB_2795644 PerCP-Cy5.5 anti-CD3 BioLegend cat. no. 100218; RRID: AB_1595492 PerCP-Cy5.5 anti-B220 BioLegend cat. no. 103236; RRID: AB_893354 APC-Fire750 anti-NK-1.1 BioLegend cat. no. 156516; RRID: AB_2892323 PE anti-CD11b BioLegend cat. no. 101207; RRID: AB_312790 APC anti-Ly6C BioLegend cat. no. 128016; RRID: AB_1732076 APC-Fire750 anti-Ly6C BioLegend cat. no. 128046; RRID: AB_2616730 BV785 anti-Ly6G BioLegend cat. no. 127645; RRID: AB_2566317 PE anti-myeloid-epithelial-reproductive tyrosine kinase (MerTK) BioLegend cat. no. 151506; RRID: AB_2617036 BV650 anti-human CD16 BioLegend cat. no. 302042; RRID: AB_2563801 APC/Fire 750 anti–I-A/I-E BioLegend cat. no. 107652; RRID: AB_2616728 BV785 anti–I-A/I-E BioLegend cat. no. 107645; RRID: AB_2565977 BV421 anti-CD16 BioLegend cat. no. 302038; RRID: AB_10898112 FITC anti-CD32a STEMCELL cat. no. 60012FI.1 APC anti-CD32b BioLegend cat. no. 398304; RRID: AB_2860997 PerCP-Cy5.5 anti-CD209 BioLegend cat. no. 330110; RRID: AB_1236468 rabbit anti-REST Invitrogen cat. no. MA5-44189; RRID: AB_2926319 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline Gibco cat. no. 14190-144 Non-fat Milk Lab Scientific bioKEMIX cat. no. M0841 SDS sample buffer Boster cat. no. AR1112 Single-cell lysis buffer Takara cat. no. 635013 RNase inhibitor Takara cat. no. 2313B Fixation Buffer BioLegend cat. no. 420801 DharmaFect3 Transfection Reagent Horizon Discovery cat. no. NC1650394 Non-essential amino acids Gibco cat. no. 11-140-050 Sodium pyruvate Gibco cat. no. 11-360-070 Macrophage-colony stimulating factor (M-CSF) Peprotech cat. no. 300-25 IL-4 Peprotech cat. no. 200-04 glycine Sigma-Aldrich cat. no. G8898 TritonX-100 Sigma-Aldrich cat. no. 93443 ProLong Gold Antifade Reagent Cell Signaling Technology cat. no. P36930 Normocin Invivogen cat. no. ant-nr-1 Blasticidin Invivogen cat. no. ant-bl-1 phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific cat. no. J63916.MCR lipopolysaccharide (LPS) eBioscience cat. no. 50-112-2025 QUANTI-Blue solution Invivogen cat. no. rep-qbs Hoechst 33342 Abcam Inc. cat. no. ab228551 Alexa Fluor 488 phalloidin Invitrogen cat. no. A12379 LIVE/DEAD Blue Fixable Dye Thermo Fisher Scientific cat. no. L34962 LIVE/DEAD Aqua Fixable Dye Thermo Fisher Scientific cat. no. L34957 Critical Commercial Assays Nano-Glo Luciferase Assay System Promega cat. no. N1120 Legendplex Bead array kits Biolegend cat. nos. .. 740390, 740451 RNeasy Micro Kit Qiagen cat. no. 74004 Gibson Assembly New England BioLabs cat. no. E2611L EasySep PE Positive Selection Kit II STEMCELL cat. no. 17684 SMART-Seq v4 Ultra Low Input RNA kit Takara cat. no. 634888 Nextera XT library preparation kit Illumina cat. no. FC-131-1096 Deposited Data Data from human PBMC of influenza patients from public datasets Liu et al.,55 Zhai et al.,56 Sweeney et al.,57 Wong et al.58–60 Ramilo et al.,61 Parnell et al.,62 Berdal et al.,63 Bermejo-Martin et al.,64 Parnell et al.,65 Zaas et al.,66 Dunning et al.,67 and Tang et al.68 GSE73072, GSE68310, GSE66099, GSE4607, GSE6269, GSE40012, GSE27131, GSE21802, GSE20346, GSE17156, GSE111368, GSE101702 Data for generating figures This paper Immport SDY2810 Experimental models: Cell lines MDCK cell BEI and Dr. Gene Tan N/A FreeStyle 293-F cells Thermo Fisher cat. no. R790-07 THP1-Blue NF-kB cells Invivogen cat. no. thp-nfkbv2 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e3

    Lysis:

    Article Title: Sialylated IgG induces the transcription factor REST in alveolar macrophages to protect against lung inflammation and severe influenza disease.
    Article Snippet: 155506; RRID: AB_2750234 BV421 anti-MerTK BioLegend cat. no. 151510; RRID: AB_2832533 APC/Fire-750 anti–major histocompatibility complex (MHC) II BioLegend cat. no. 107652; RRID: AB_2616728 APC anti-CD209b Invitrogen cat. no. 17-2093-82; RRID: AB_11151692 BUV395 anti-CD24 BD Horizon cat. no. 566221; RRID: AB_2632389 BUV805 anti-CD103 BD Horizon cat. no. 741948; RRID: AB_2871259 PE-Cy7 anti-CD32b BioLegend cat. no. 303213; RRID: AB_2616922 PEDazzle 594 anti-CD64 BioLegend cat. no. 305031; RRID: AB_2564186 BUV615 anti-CD16 BD Horizon cat. no. 751572; RRID: AB_2875567 Human anti-kappa Southern Biotech cat. no. 9230-01; RRID: AB_2796705 Human anti-lambda Southern Biotech cat. no. 9180-01; RRID: AB_2796674 HRP–conjugated anti-human IgG secondary antibody Southern Biotech cat. no. 2040-05: RRID: AB_2795644 PerCP-Cy5.5 anti-CD3 BioLegend cat. no. 100218; RRID: AB_1595492 PerCP-Cy5.5 anti-B220 BioLegend cat. no. 103236; RRID: AB_893354 APC-Fire750 anti-NK-1.1 BioLegend cat. no. 156516; RRID: AB_2892323 PE anti-CD11b BioLegend cat. no. 101207; RRID: AB_312790 APC anti-Ly6C BioLegend cat. no. 128016; RRID: AB_1732076 APC-Fire750 anti-Ly6C BioLegend cat. no. 128046; RRID: AB_2616730 BV785 anti-Ly6G BioLegend cat. no. 127645; RRID: AB_2566317 PE anti-myeloid-epithelial-reproductive tyrosine kinase (MerTK) BioLegend cat. no. 151506; RRID: AB_2617036 BV650 anti-human CD16 BioLegend cat. no. 302042; RRID: AB_2563801 APC/Fire 750 anti–I-A/I-E BioLegend cat. no. 107652; RRID: AB_2616728 BV785 anti–I-A/I-E BioLegend cat. no. 107645; RRID: AB_2565977 BV421 anti-CD16 BioLegend cat. no. 302038; RRID: AB_10898112 FITC anti-CD32a STEMCELL cat. no. 60012FI.1 APC anti-CD32b BioLegend cat. no. 398304; RRID: AB_2860997 PerCP-Cy5.5 anti-CD209 BioLegend cat. no. 330110; RRID: AB_1236468 rabbit anti-REST Invitrogen cat. no. MA5-44189; RRID: AB_2926319 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline Gibco cat. no. 14190-144 Non-fat Milk Lab Scientific bioKEMIX cat. no. M0841 SDS sample buffer Boster cat. no. AR1112 Single-cell lysis buffer Takara cat. no. 635013 RNase inhibitor Takara cat. no. 2313B Fixation Buffer BioLegend cat. no. 420801 DharmaFect3 Transfection Reagent Horizon Discovery cat. no. NC1650394 Non-essential amino acids Gibco cat. no. 11-140-050 Sodium pyruvate Gibco cat. no. 11-360-070 Macrophage-colony stimulating factor (M-CSF) Peprotech cat. no. 300-25 IL-4 Peprotech cat. no. 200-04 glycine Sigma-Aldrich cat. no. G8898 TritonX-100 Sigma-Aldrich cat. no. 93443 ProLong Gold Antifade Reagent Cell Signaling Technology cat. no. P36930 Normocin Invivogen cat. no. ant-nr-1 Blasticidin Invivogen cat. no. ant-bl-1 phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific cat. no. J63916.MCR lipopolysaccharide (LPS) eBioscience cat. no. 50-112-2025 QUANTI-Blue solution Invivogen cat. no. rep-qbs Hoechst 33342 Abcam Inc. cat. no. ab228551 Alexa Fluor 488 phalloidin Invitrogen cat. no. A12379 LIVE/DEAD Blue Fixable Dye Thermo Fisher Scientific cat. no. L34962 LIVE/DEAD Aqua Fixable Dye Thermo Fisher Scientific cat. no. L34957 Critical Commercial Assays Nano-Glo Luciferase Assay System Promega cat. no. N1120 Legendplex Bead array kits Biolegend cat. nos. .. 740390, 740451 RNeasy Micro Kit Qiagen cat. no. 74004 Gibson Assembly New England BioLabs cat. no. E2611L EasySep PE Positive Selection Kit II STEMCELL cat. no. 17684 SMART-Seq v4 Ultra Low Input RNA kit Takara cat. no. 634888 Nextera XT library preparation kit Illumina cat. no. FC-131-1096 Deposited Data Data from human PBMC of influenza patients from public datasets Liu et al.,55 Zhai et al.,56 Sweeney et al.,57 Wong et al.58–60 Ramilo et al.,61 Parnell et al.,62 Berdal et al.,63 Bermejo-Martin et al.,64 Parnell et al.,65 Zaas et al.,66 Dunning et al.,67 and Tang et al.68 GSE73072, GSE68310, GSE66099, GSE4607, GSE6269, GSE40012, GSE27131, GSE21802, GSE20346, GSE17156, GSE111368, GSE101702 Data for generating figures This paper Immport SDY2810 Experimental models: Cell lines MDCK cell BEI and Dr. Gene Tan N/A FreeStyle 293-F cells Thermo Fisher cat. no. R790-07 THP1-Blue NF-kB cells Invivogen cat. no. thp-nfkbv2 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e3

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Transfection:

    Article Title: Sialylated IgG induces the transcription factor REST in alveolar macrophages to protect against lung inflammation and severe influenza disease.
    Article Snippet: 155506; RRID: AB_2750234 BV421 anti-MerTK BioLegend cat. no. 151510; RRID: AB_2832533 APC/Fire-750 anti–major histocompatibility complex (MHC) II BioLegend cat. no. 107652; RRID: AB_2616728 APC anti-CD209b Invitrogen cat. no. 17-2093-82; RRID: AB_11151692 BUV395 anti-CD24 BD Horizon cat. no. 566221; RRID: AB_2632389 BUV805 anti-CD103 BD Horizon cat. no. 741948; RRID: AB_2871259 PE-Cy7 anti-CD32b BioLegend cat. no. 303213; RRID: AB_2616922 PEDazzle 594 anti-CD64 BioLegend cat. no. 305031; RRID: AB_2564186 BUV615 anti-CD16 BD Horizon cat. no. 751572; RRID: AB_2875567 Human anti-kappa Southern Biotech cat. no. 9230-01; RRID: AB_2796705 Human anti-lambda Southern Biotech cat. no. 9180-01; RRID: AB_2796674 HRP–conjugated anti-human IgG secondary antibody Southern Biotech cat. no. 2040-05: RRID: AB_2795644 PerCP-Cy5.5 anti-CD3 BioLegend cat. no. 100218; RRID: AB_1595492 PerCP-Cy5.5 anti-B220 BioLegend cat. no. 103236; RRID: AB_893354 APC-Fire750 anti-NK-1.1 BioLegend cat. no. 156516; RRID: AB_2892323 PE anti-CD11b BioLegend cat. no. 101207; RRID: AB_312790 APC anti-Ly6C BioLegend cat. no. 128016; RRID: AB_1732076 APC-Fire750 anti-Ly6C BioLegend cat. no. 128046; RRID: AB_2616730 BV785 anti-Ly6G BioLegend cat. no. 127645; RRID: AB_2566317 PE anti-myeloid-epithelial-reproductive tyrosine kinase (MerTK) BioLegend cat. no. 151506; RRID: AB_2617036 BV650 anti-human CD16 BioLegend cat. no. 302042; RRID: AB_2563801 APC/Fire 750 anti–I-A/I-E BioLegend cat. no. 107652; RRID: AB_2616728 BV785 anti–I-A/I-E BioLegend cat. no. 107645; RRID: AB_2565977 BV421 anti-CD16 BioLegend cat. no. 302038; RRID: AB_10898112 FITC anti-CD32a STEMCELL cat. no. 60012FI.1 APC anti-CD32b BioLegend cat. no. 398304; RRID: AB_2860997 PerCP-Cy5.5 anti-CD209 BioLegend cat. no. 330110; RRID: AB_1236468 rabbit anti-REST Invitrogen cat. no. MA5-44189; RRID: AB_2926319 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline Gibco cat. no. 14190-144 Non-fat Milk Lab Scientific bioKEMIX cat. no. M0841 SDS sample buffer Boster cat. no. AR1112 Single-cell lysis buffer Takara cat. no. 635013 RNase inhibitor Takara cat. no. 2313B Fixation Buffer BioLegend cat. no. 420801 DharmaFect3 Transfection Reagent Horizon Discovery cat. no. NC1650394 Non-essential amino acids Gibco cat. no. 11-140-050 Sodium pyruvate Gibco cat. no. 11-360-070 Macrophage-colony stimulating factor (M-CSF) Peprotech cat. no. 300-25 IL-4 Peprotech cat. no. 200-04 glycine Sigma-Aldrich cat. no. G8898 TritonX-100 Sigma-Aldrich cat. no. 93443 ProLong Gold Antifade Reagent Cell Signaling Technology cat. no. P36930 Normocin Invivogen cat. no. ant-nr-1 Blasticidin Invivogen cat. no. ant-bl-1 phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific cat. no. J63916.MCR lipopolysaccharide (LPS) eBioscience cat. no. 50-112-2025 QUANTI-Blue solution Invivogen cat. no. rep-qbs Hoechst 33342 Abcam Inc. cat. no. ab228551 Alexa Fluor 488 phalloidin Invitrogen cat. no. A12379 LIVE/DEAD Blue Fixable Dye Thermo Fisher Scientific cat. no. L34962 LIVE/DEAD Aqua Fixable Dye Thermo Fisher Scientific cat. no. L34957 Critical Commercial Assays Nano-Glo Luciferase Assay System Promega cat. no. N1120 Legendplex Bead array kits Biolegend cat. nos. .. 740390, 740451 RNeasy Micro Kit Qiagen cat. no. 74004 Gibson Assembly New England BioLabs cat. no. E2611L EasySep PE Positive Selection Kit II STEMCELL cat. no. 17684 SMART-Seq v4 Ultra Low Input RNA kit Takara cat. no. 634888 Nextera XT library preparation kit Illumina cat. no. FC-131-1096 Deposited Data Data from human PBMC of influenza patients from public datasets Liu et al.,55 Zhai et al.,56 Sweeney et al.,57 Wong et al.58–60 Ramilo et al.,61 Parnell et al.,62 Berdal et al.,63 Bermejo-Martin et al.,64 Parnell et al.,65 Zaas et al.,66 Dunning et al.,67 and Tang et al.68 GSE73072, GSE68310, GSE66099, GSE4607, GSE6269, GSE40012, GSE27131, GSE21802, GSE20346, GSE17156, GSE111368, GSE101702 Data for generating figures This paper Immport SDY2810 Experimental models: Cell lines MDCK cell BEI and Dr. Gene Tan N/A FreeStyle 293-F cells Thermo Fisher cat. no. R790-07 THP1-Blue NF-kB cells Invivogen cat. no. thp-nfkbv2 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e3

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Luciferase:

    Article Title: Sialylated IgG induces the transcription factor REST in alveolar macrophages to protect against lung inflammation and severe influenza disease.
    Article Snippet: 155506; RRID: AB_2750234 BV421 anti-MerTK BioLegend cat. no. 151510; RRID: AB_2832533 APC/Fire-750 anti–major histocompatibility complex (MHC) II BioLegend cat. no. 107652; RRID: AB_2616728 APC anti-CD209b Invitrogen cat. no. 17-2093-82; RRID: AB_11151692 BUV395 anti-CD24 BD Horizon cat. no. 566221; RRID: AB_2632389 BUV805 anti-CD103 BD Horizon cat. no. 741948; RRID: AB_2871259 PE-Cy7 anti-CD32b BioLegend cat. no. 303213; RRID: AB_2616922 PEDazzle 594 anti-CD64 BioLegend cat. no. 305031; RRID: AB_2564186 BUV615 anti-CD16 BD Horizon cat. no. 751572; RRID: AB_2875567 Human anti-kappa Southern Biotech cat. no. 9230-01; RRID: AB_2796705 Human anti-lambda Southern Biotech cat. no. 9180-01; RRID: AB_2796674 HRP–conjugated anti-human IgG secondary antibody Southern Biotech cat. no. 2040-05: RRID: AB_2795644 PerCP-Cy5.5 anti-CD3 BioLegend cat. no. 100218; RRID: AB_1595492 PerCP-Cy5.5 anti-B220 BioLegend cat. no. 103236; RRID: AB_893354 APC-Fire750 anti-NK-1.1 BioLegend cat. no. 156516; RRID: AB_2892323 PE anti-CD11b BioLegend cat. no. 101207; RRID: AB_312790 APC anti-Ly6C BioLegend cat. no. 128016; RRID: AB_1732076 APC-Fire750 anti-Ly6C BioLegend cat. no. 128046; RRID: AB_2616730 BV785 anti-Ly6G BioLegend cat. no. 127645; RRID: AB_2566317 PE anti-myeloid-epithelial-reproductive tyrosine kinase (MerTK) BioLegend cat. no. 151506; RRID: AB_2617036 BV650 anti-human CD16 BioLegend cat. no. 302042; RRID: AB_2563801 APC/Fire 750 anti–I-A/I-E BioLegend cat. no. 107652; RRID: AB_2616728 BV785 anti–I-A/I-E BioLegend cat. no. 107645; RRID: AB_2565977 BV421 anti-CD16 BioLegend cat. no. 302038; RRID: AB_10898112 FITC anti-CD32a STEMCELL cat. no. 60012FI.1 APC anti-CD32b BioLegend cat. no. 398304; RRID: AB_2860997 PerCP-Cy5.5 anti-CD209 BioLegend cat. no. 330110; RRID: AB_1236468 rabbit anti-REST Invitrogen cat. no. MA5-44189; RRID: AB_2926319 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline Gibco cat. no. 14190-144 Non-fat Milk Lab Scientific bioKEMIX cat. no. M0841 SDS sample buffer Boster cat. no. AR1112 Single-cell lysis buffer Takara cat. no. 635013 RNase inhibitor Takara cat. no. 2313B Fixation Buffer BioLegend cat. no. 420801 DharmaFect3 Transfection Reagent Horizon Discovery cat. no. NC1650394 Non-essential amino acids Gibco cat. no. 11-140-050 Sodium pyruvate Gibco cat. no. 11-360-070 Macrophage-colony stimulating factor (M-CSF) Peprotech cat. no. 300-25 IL-4 Peprotech cat. no. 200-04 glycine Sigma-Aldrich cat. no. G8898 TritonX-100 Sigma-Aldrich cat. no. 93443 ProLong Gold Antifade Reagent Cell Signaling Technology cat. no. P36930 Normocin Invivogen cat. no. ant-nr-1 Blasticidin Invivogen cat. no. ant-bl-1 phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific cat. no. J63916.MCR lipopolysaccharide (LPS) eBioscience cat. no. 50-112-2025 QUANTI-Blue solution Invivogen cat. no. rep-qbs Hoechst 33342 Abcam Inc. cat. no. ab228551 Alexa Fluor 488 phalloidin Invitrogen cat. no. A12379 LIVE/DEAD Blue Fixable Dye Thermo Fisher Scientific cat. no. L34962 LIVE/DEAD Aqua Fixable Dye Thermo Fisher Scientific cat. no. L34957 Critical Commercial Assays Nano-Glo Luciferase Assay System Promega cat. no. N1120 Legendplex Bead array kits Biolegend cat. nos. .. 740390, 740451 RNeasy Micro Kit Qiagen cat. no. 74004 Gibson Assembly New England BioLabs cat. no. E2611L EasySep PE Positive Selection Kit II STEMCELL cat. no. 17684 SMART-Seq v4 Ultra Low Input RNA kit Takara cat. no. 634888 Nextera XT library preparation kit Illumina cat. no. FC-131-1096 Deposited Data Data from human PBMC of influenza patients from public datasets Liu et al.,55 Zhai et al.,56 Sweeney et al.,57 Wong et al.58–60 Ramilo et al.,61 Parnell et al.,62 Berdal et al.,63 Bermejo-Martin et al.,64 Parnell et al.,65 Zaas et al.,66 Dunning et al.,67 and Tang et al.68 GSE73072, GSE68310, GSE66099, GSE4607, GSE6269, GSE40012, GSE27131, GSE21802, GSE20346, GSE17156, GSE111368, GSE101702 Data for generating figures This paper Immport SDY2810 Experimental models: Cell lines MDCK cell BEI and Dr. Gene Tan N/A FreeStyle 293-F cells Thermo Fisher cat. no. R790-07 THP1-Blue NF-kB cells Invivogen cat. no. thp-nfkbv2 (Continued on next page) Immunity 58, 182–196.e1–e10, January 14, 2025 e3

    Control:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    shRNA:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    In Situ:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Proximity Ligation Assay:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Bradford Protein Assay:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Extraction:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    cDNA Synthesis:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Real-time Polymerase Chain Reaction:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    SYBR Green Assay:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    WST-1 Assay:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Reporter Assay:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Co-Immunoprecipitation Assay:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Chromatin Immunoprecipitation:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Western Blot:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Multiple Displacement Amplification:

    Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 (E1L3N) Cell Signaling Technology Cat#13684; RRID:AB_2799389 YAP (D8H1X) Cell Signaling Technology Cat#14074; RRID:AB_2798692 YAP (63.7) Santa Cruz Biotechnology Cat#sc-101199; RRID: AB_1131430 Phospho-YAP (Ser127) (D9W2I) Cell Signaling Technology Cat#13008; RRID:AB_2650553 Pan-TEAD Cell Signaling Technology Cat#13295; RRID:AB_2687902 TEAD4 antibody (5H3) AbCam Cat#ab58310; RRID: AB_945789 TAZ Cell Signaling Technology Cat#83669; RRID:AB_2800026 Karyopherin α4 AbCam Cat#ab6039; RRID:AB_305248 Karyopherin β1 (3E9) AbCam Cat#ab2811; RRID:AB_2133989 COX-2 AbCam Cat#ab15191; RRID:AB_2085144 EP4 (C-4) Santa Cruz Biotechnology Cat#sc-55596; RRID:AB_2174775 Histone 3 (1G1) Santa Cruz Biotechnology Cat#sc-517576; RRID:AB_2848194 Alpha tubulin (DM1A) Santa Cruz Biotechnology Cat#sc-32293; RRID:AB_628412 Vinculin (7F9) Santa Cruz Biotechnology Cat#sc-73614; RRID:AB_1131294 Lamin B1 (B-10) Santa Cruz Biotechnology Cat#sc-374015; RRID:AB_1237141 BrdU (IIB5) Santa Cruz Biotechnology Cat#sc-32323; RRID:AB_626766 Goat anti-Rabbit IgG (H + L), Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Goat anti-mouse IgG (H + L), Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11032; RRID:AB_2534091 Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0448; RRID:AB_2617138 Goat Anti-Mouse Immunoglobulins/HRP Dako Cat#P0447; RRID:AB_2617137 Rabbit TrueBlot ULTRA: Anti-Rabbit IgG HRP Rockland Immunochemicals Cat#18-8816-33; RRID:AB_469529 Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP Rockland Immunochemicals Cat#18-8817-33; RRID:AB_10126892 Chemicals, peptides, and recombinant proteins 16, 16-dimethyl Prostaglandin E2 (dmPGE2) Cayman Chemical Cat#14750 AH6809 Cayman Chemical Cat#14050 ONO-AE3-208 Cayman Chemical Cat#14522 Celecoxib Selleck Chemicals Cat#S1261 Verteporfin Selleck Chemicals Cat#S1786 Tween 20 Sigma Cat#P1379-25ML Triton X-100 Sigma Cat#×100-500ML Crystal Violet Sigma Cat#C0775-25G DAPI Sigma Cat#D9542-5MG Fetal Bovine Serum HyClone Cat#SV30160 RPMI 1640 HyClone Cat#SH30027 MEM HyClone Cat#SH30024 DMEM HyClone Cat#SH30243 Penicillin-Streptomycin (10,000 U/mL) HyClone Cat#SV30010 Non-Essential Amino Acids HyClone Cat#SH30238.01 GlutaMax Thermo Fisher Scientific Cat#35050061 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat#25200056 (Continued on next page) Cell Reports 45, 116963, February 24, 2026 11 .. REAGENT or RESOURCE SOURCE IDENTIFIER Opti-MEM Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific CatJ19943.K2 Lipofectamine 3000 Transfection Reagent Thermo Fisher Scientific Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 HALT protease/phosphatase inhibitor cocktail Thermo Fisher Scientific Cat#78440 RIPA Lysis buffer Thermo Fisher Scientific Cat#89900 IP Lysis Buffer Thermo Fisher Scientific Cat#87787 Phosphate Buffered Saline (PBS), pH 7.4 Thermo Fisher Scientific Cat#10010023 Tris-buffered Saline (TBS), pH 7.4 Thermo Fisher Scientific Cat#J62938.K7 BrdU (5-Bromo-2′-Deoxyuridine) Thermo Fisher Scientific Cat#B23151 Control siRNA Dharmacon Cat#D-001810-10-20 CD274 siRNA Dharmacon Cat#L-015836-01-0010 KPNA4 siRNA Dharmacon Cat#L-017477-00-0010 KPNB1 siRNA Dharmacon Cat#L-017523-00-0010 WWTR1 siRNA Dharmacon Cat#L-016083-00-0010 Control shRNA Santa Cruz Biotechnology Cat#sc-108060 YAP1 shRNA Santa Cruz Biotechnology Cat#sc-38637-SH Protein A/G PLUS-Agarose Santa Cruz Biotechnology Cat#sc-2003 TrueBlot Anti-Rabbit Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8800-25 TrueBlot Anti-Mouse Ig IP Agarose Beads Rockland Immunochemicals Cat#00-8811-25 CMV-Renilla Addgene Cat#118066 8xGTIIC Addgene Cat#34615 Critical commercial assays Duolink In Situ Detection Reagents Orange Sigma Cat#DUO92007 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Sigma Cat#DUO92002 Duolink In Situ PLA® Probe Anti-Mouse MINUS Sigma Cat#DUO92004 Pierce Bradford Protein Assay Kit Thermo Fisher Scientific Cat#23200 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat#78835 RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Fisher Scientific Cat#K1632 Maxima Probe/ROX qPCR Master Mix (2×) Thermo Fisher Scientific Cat#0232 SYBR Green Universal Master Mix Thermo Fisher Scientific Cat#4309155 WST-1 Assay Reagent AbCam Cat#ab155902 Dual-luciferase Reporter Assay System Promega Cat#E1910 Nuclear Extract Kit Active Motif Cat#40410 Nuclear Complex Co-IP Kit Active Motif Cat#54001 ChIP-IT Express Enzymatic kit Active Motif Cat#53009 Immobilon ECL Ultra Western HRP Substrate Millipore Cat#WBULS0500 MycoAlert® Mycoplasma Detection Kit Lonza Cat#LT07-418 Experimental models: Cell lines RKO ATCC Cat#CRL-2577; RRID:CVCL_0504 SW480 ATCC Cat#CCL-228; RRID:CVCL_0546 MDA-MB-231 ATCC Cat#HTB-26; RRID:CVCL_0062 (Continued on next page) 12 Cell Reports 45, 116963, February 24, 2026 ..

    Cell Culture:

    Article Title: An Orthotopic Vestibular Schwannoma Mouse Model to Study Tumor-Host Interactions and Mechanism of Sensorineural Hearing Loss.
    Article Snippet: .. At our institution, the cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; high glucose, with L-glutamine, sodium pyruvate, and phenol red; without HEPES) (Thermo Fisher Scientific, Cat. No. 11995073) supplemented with 10 % fetal bovine serum (FBS) (Thermo Fisher Scientific, Cat. No. 16000044), 1 % penicillin/streptomycin (Sigma-Aldrich, Cat. No. P4333) and 10 μM forskolin (Sigma-Aldrich, Cat. No. 344270) at 37◦C in a humidified atmosphere with 5 % CO2. .. Cell cultures were routinely tested for mycoplasma contamination (MycoAlert, Lonza Biosciences, Cat. No. LTO7).

    other:




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